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KMID : 0644120100230030026
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Journal of Oriental Gynecology
2010 Volume.23 No. 3 p.26 ~ p.37
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Anti-oxidant Effects of Samultang-Gami on MEF Cells
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Jung Jae-Joong
Goo Sun-Young
Go Eun-Bi
Sung Jung-Suk
Kim Dong-Il
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Abstract
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Purpose: This experiment is designed to find out anti-oxidant effects of Samultang-Gami which was composed of Rehmanniae Radix(RR), Angelicae Gigantis Radix, Cnidii Rhizoma(CR), Paeoniae Radix(PR), Cortex Moutan Radicis, Hedyotis Diffusa(HD) and Caesalpinia Sappan on MEF cells.
Methods: In vitro antioxidant effects were measured by MTT assay, DPPH assay, cell cycle analysis, AnnexinV-FITC/PI assay and DAPI staining using MEF cells treated with various concentrations of 70% ethanol extract of Samultang-Gami.
Results: 1. In the scavenging for DPPH radical, the each treated groups of PR, CR and HD showed positive effects. RR and CR increased the viability of oxidative damaged MEF cells in a dose-dependent manner. 2. 70% ethanol extract of Samultang-Gami was shown best antioxidative effect in the concentration of . 3. The treatment of Samultang-Gami in oxidative damaged MEF cells didn’t have any effect on cell cycle restoration. but it could lower late apoptosis rate a little and be observed the protection of nucleus.
Conclusion: It can be concluded that Samultang-Gami, RR and CR have antioxidant effects on MEF cells.
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KEYWORD
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Samultang-Gami, MEF cells, Anti-oxidant
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